The method consists in extracting of the compound with ice-cold perchloric acid solution, centrifuging, decanting and filtrating, followed by hydrolysis of the compound in a portion of the filtrate with potassium hydroxide into gluconate. Transformation of the gluconate in the extract by the following reactions: 1) with adenosine-5-triphosphate (ATP) and 2) with concomitant reduction of an equivalent amount of nicotinamide adenine dinucleotide phosphate (NADP). Photometric measurement of the amount of nicotinamide adenine dinucleotide phosphate (reduced) (NADPH) formed.
Status : WithdrawnPublication date : 1979-02
Edition : 1Number of pages : 4
Technical Committee:Meat, poultry, fish, eggs and their products